Gold Nanoparticle Product Selection Guide
Choose a Gold Nanoparticle Product Based on Your Particular Application
|Application||Gold Nanoparticle Size Range||Surface Chemistry||Benefits|
|Protein Conjugation||5nm-100nm||Standard (citrate)||Quick|
|NHS||Covalent conjugation to primary amines, increased conjugate stability, less non-specific protein binding.|
Covalent conjugation to thiols, increased conjugate stability, less non-specific protein binding.
Covalent conjugation to primary amines, increased conjugate stability, less non-specific protein binding.
|Amine||Conjugation of NHS and carboxyl ligands.|
|Streptavidin||Conjugation to biotinylated ligands.|
|Modification with thiolated ligands||5nm-100nm||Standard (citrate-coated)||Classic starting material, no additional stabilizers added.|
|Stabilized (surfactant)||Increased stability during functionalization but reduced binding kinetics.|
|Oligonucleotide Conjugation||5nm-20nm||Standard (citrate)||Ideal for conjugation of thiol-modified oligos to small particle sizes (5nm-20nm). Does not work well for larger particles.|
|5nm-100nm||OligoREADY||Ideal for conjugation of thiol-modified oligos directly to the gold surface.|
|5nm-100nm||NHS||Ideal for covalent conjugation of amine-modified oligos. Final conjugate will have a PEG-linker between oligo and gold surface.|
|5nm-100nm||Maleimide||Ideal for covalent conjugation of thiol-modified oligos.
Final conjugate will have a PEG-linker between oligo and gold surface.
|Immunoblotting/Western Blot||5nm-20nm||Secondary Antibody Gold Conjugates||Colorimetric detection. Permanent label|
|Immunohistochemistry||5nm-40nm||Secondary Antibody Gold Conjugates||High contrast label|
|Cellular Uptake||30nm-80nm||Transferrin Gold Conjugates||Active uptake through endocytosis|
|Standard (citrate-coated)||Non-specific cellular uptake|
|Darkfield Microscopy||50nm-100nm||Gold Conjugates|
|Lateral Flow/Dip-Stick Assays||20nm-80nm||Standard (citrate-coated)||Ideal for generation of gold conjugates through passive adsorption of antibodies to the gold nanoparticle surface.|
|NHS||Ideal for covalent conjugation of antibodies to gold nanoparticles.|
|Maleimide||Ideal for conjugation of thiol-modified ligands to gold nanoparticles.|
|Gold Conjugates||Pre-made secondary antibody conjugates|
|Vertical Flow||20nm-40nm||Standard (citrate-coated)||
Ideal for generation of gold conjugates through passive adsorption of antibodies to the gold nanoparticle surface.
Ideal for covalent conjugation of antibodies to gold nanoparticles.
Ideal for conjugation of thiol-modified ligands to gold nanoparticles.
|Gold Conjugates||Pre-made secondary antibody conjugates.|
|Tumor Targeting||20nm-80nm||Methyl (methoxy) Gold Nanoparticles||Can in some cases be used for passive targeting of certain tumors in vivo. Inert material with low non-specific protein binding in serum.|
|Light Microscopy||5nm-10nm||Gold Conjugates||Ability to label tissue sections for both light and electron microscopy. Alternative to peroxidase and PAP-based stains. Sensitivity can be enhanced with silver enhancement techniques.|
|ELISA||5nm-30nm||Gold Conjugates||Colorimetric Detection|