Protein Conjugation Services - Nanoparticles

Cytodiagnostics provides custom conjugation of full length antibodies, antibody fragments (e.g. Fab', F(ab')2, scFv), enzymes and other proteins to our gold nanoparticles, silver nanoparticles, and gold nanourchins with a wide range of available sizes (up to 100nm in diameter).

Conjugation can either be done through direct attachment to the nanoparticle surface (standard), or covalently through a linker, depending on customer need. 

To be able to perform your custom conjugation we require 2-3 mg of purified lyophilized protein. If you want us to source your protein of antibody we would be glad to assist.

A successful conjugation would result in a typical yield of 10ml* of your protein gold, silver nanoparticle or gold urchin conjugate at an optical density of 3 (OD=3). This amount is generally sufficient to probe 10-15 PVDF or nitrocellulose membranes or more using 15ml of a 1:15 diluted conjugate per strip as a starting point. 

Our services include:

  1. Sourcing of your antibody or protein if needed (this service is not included in price below).
  2. Conjugation of your protein to either gold, silver or gold nanourchin nanoparticles with the size of your choice.
  3. Purification of conjugate.
  4. Functionality testing (upon request)

Contact Us to Order: Price (USD) $1850

If you prefer to perform the conjugation on your own, try our Gold Nanoparticle Conjugation Kits,  Silver Nanoparticle Conjugation Kits or Gold NanoUrchin Conjugation Kits


*Due to variations in affinity between different proteins and the nanoparticle surface, the yield stated above is just an average, and not a guaranteed final volume of product.