Protein Conjugation Services - Nanoparticles
Cytodiagnostics provides custom conjugation of full length antibodies, antibody fragments (e.g. Fab', F(ab')2, scFv), enzymes and other proteins to our gold nanoparticles, silver nanoparticles, and gold nanourchins with a wide range of available sizes (up to 100nm in diameter).
Conjugation can either be done through direct attachment to the nanoparticle surface (standard), or covalently through a linker, depending on customer need.
To be able to perform your custom conjugation we require 2-3 mg of purified lyophilized protein. If you want us to source your protein of antibody we would be glad to assist.
A successful conjugation would result in a typical yield of 10ml* of your protein gold, silver nanoparticle or gold urchin conjugate at an optical density of 3 (OD=3). This amount is generally sufficient to probe 10-15 PVDF or nitrocellulose membranes or more using 15ml of a 1:15 diluted conjugate per strip as a starting point.
Our services include:
- Sourcing of your antibody or protein if needed (this service is not included in price below).
- Conjugation of your protein to either gold, silver or gold nanourchin nanoparticles with the size of your choice.
- Purification of conjugate.
- Functionality testing (upon request)
Contact Us to Order: Price (USD) $1850
If you prefer to perform the conjugation on your own, try our Gold Nanoparticle Conjugation Kits, Silver Nanoparticle Conjugation Kits or Gold NanoUrchin Conjugation Kits.
*Due to variations in affinity between different proteins and the nanoparticle surface, the yield stated above is just an average, and not a guaranteed final volume of product.