Human VEGF ELISA Kit

Product overview:

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a 45 kDa homodimeric, disulfide-linked glycoprotein produced by many cell types that facilitates angiogenesis and vasculogenesis.

The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E (Orf-VEGF), and PIGF (placental growth factor). There are multiple isoforms of VEGF-A expressed from alternative splicing of mRNA from a single VEGFA gene, among which VEGF165 is the most abundant isoform and essential for angiogenesis.

VEGF regulates a variety of functions, including proliferation and survival of endothelial cells, vascular permeability, cell migration in macrophage lineage, wound healing, bone formation, and development of embryonic vasculature. VEGF-A activates two receptors, VEGFR-1, and VEGFR-2, to regulate angiogenesis and vascular permeability, while VEGF-C and VEGF-D bind their receptor, VEGFR-3, mainly regulating lymphangiogenesis. VEGF expression is induced by hypoxia and cytokines like IL-1, IL-6, IL-8, and TNF-alpha.

Our ELISA kit has several benefits:

  • Ready to use. Simply add standards and samples directly to wells. No need for lengthy blocking or rehydration steps.
  • High sensitivity, specificity and reproducibility.
  • Fully validated in the sample types listed below.
  • 96-well plate is breakable into 12 x 8 well strips.

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 Product Specification

Protein name

VEGF

Alternate names

Vascular endothelial growth factor A, vascular endothelial growth factor A121, vascular endothelial growth factor A165, Vascular permeability factor, VEGF-A, VPF

Species reactivity

Human

Assay format

Solid-phase Sandwich ELISA (quantitative)

Sample type

Serum, Cell culture supernatant

Sample volume

100 μL

Assay length

4.5 hrs

Analytical sensitivity

<30 pg/mL

Assay range

31.25 – 2000 pg/mL

Intra-assay CV

<8%

Inter-assay CV

<10%

Serum recovery

95.3%

Detection & Instrument

Colorimetric, Microplate Reader

UniProt ID

(Human) P15692