Human IL-6 ELISA Kit

Product overview:

Interleukin-6 (IL-6), a 26 kDa glycoprotein composing of 184 amino acids, plays a pivotal role in inflammation, immune responses, hematopoiesis, and oncogenesis. While its typical circulating level in healthy adults is less than 5 pg/mL, they escalate rapidly during inflammation. IL-6 is expressed in diverse cell types, including adipocytes, fibroblasts, osteoblasts, neurons, and immune cells. Elevated IL-6 levels are associated with conditions like cardiovascular disease, insulin resistance, type 2 diabetes, asthma, rheumatoid arthritis, and advanced-stage cancer.

As a signaling molecule, IL-6 regulates cell growth and differentiation, particularly in immune responses and acute phase reactions. Secreted by various cell types, including T cells and macrophages, IL-6 exerts its actions through a heterodimeric receptor composed of IL-6R and glycoprotein 130 (gp130). This receptor complex transduces signals, contributing to immune responses, hematopoiesis, bone metabolism, and cancer progression.

IL-6 is not constitutively produced but is induced by factors like cytokines, lipopolysaccharide, or viral infections. Its broad tissue effects vary from inducing growth and inhibition to differentiation, depending on the target cells. Abnormal IL-6 production is implicated in various diseases, and measuring its levels in serum provides diagnostic insights. The multifaceted nature of IL-6 and its involvement in diverse physiological processes make it a central figure in immunology, with significant implications for both research and clinical applications.

Our ELISA kit has several benefits:

  • Ready to use. Simply add standards and samples directly to wells. No need for lengthy blocking or rehydration steps.
  • High sensitivity, specificity and reproducibility.
  • Fully validated in the sample types listed below.
  • 96-well plate is breakable into 12 x 8 well strips.

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 Product Specification

Protein name

IL-6

Alternate names

Interleukin-6, B-cell differentiation factor 2, B-cell stimulatory factor 2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2

Species reactivity

Human

Assay format

Solid-phase Sandwich ELISA (quantitative)

Sample type

Serum, Plasma, Cell culture supernatant

Sample volume

100 μL

Assay length

4.5 hrs

Analytical sensitivity

<5 pg/mL

Assay range

7.8 – 500 pg/mL

Intra-assay CV

<8%

Inter-assay CV

<8%

Recovery (%)

Serum: 103.8
Plasma: 95.5
Cell culture media: 94.4

Detection & Instrument

Colorimetric, Microplate Reader

UniProt ID

(Human) P05231