5nm AptamerREADY Gold Nanoparticle Conjugation Kit (10 reactions) media thumbnails
5nm AptamerREADY Gold Nanoparticle Conjugation Kit (10 reactions)-1
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5nm AptamerREADY Gold Nanoparticle Conjugation Kit (10 reactions)

SKU: AGC-5-2

$465.00

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Purification: No Spin Column

Cytodiagnostics AptamerREADY™ Gold Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of thiolated aptamers directly to the gold surface. 

The kit contains ready-to-use pre-made mixtures. No activation, manipulation or time consuming "salt-aging" steps are required for conjugation. Simply mix your thiol-modified aptamer with the pre-activated gold nanoparticles supplied in the kit. Conjugation of the aptamer is achieved by the formation of a strong and stable gold-thiol bond. 

Kit Components

  • 10 vials of 5nm AptamerREADY™ Gold Nanoparticles (lyophilized) - Sufficient for generation of 10 x 0.2 ml gold conjugate at OD=10

Features & Benefits

  • Results in thiol-aptamer bound directly to the gold surface through a stable thiol-gold bond.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Does not require time consuming salt aging procedures saving you valuable research time.

Gold Nanoparticle Specifications

Gold Surface: Proprietary AptamerREADY™-coating

Core diameter: 5nm 

Concentration: OD=2 (when dissolved a final volume of 1 ml)

Particles per ml: ~1.09E+14 (when dissolved to a final volume of 1 ml)

Absorbance (lambda max): 515-520nm

5nm gold nanoparticles UV-VIS Spectrum

Diameter  Peak SPR Wavelength (nm) NPS/ml* Wt. Conc. (mg/ml)* Molar Ext. (M-1cm-1) Size Dispersity (+/-nm) Particle Volume (nm3) Surface Area (nm2) Surface/Volume Ratio Particle Mass (g) Molar Mass (g/mol) Molar Concentration*
5 515-520 5.47E+13 6.94E-02 1.10E+07 <15% 6.54E+01 7.85E+01 1.2 1.27E-18 7.64E+05 9.08E-08

* All concentrations as indicated at an optical density of 1 (OD=1, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)