50nm OligoREADY Gold Nanoparticle Conjugation Kit (3 reactions)

SKU: OGC-50-1

$185.00

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Cytodiagnostics OligoREADY™ Gold Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of thiolated-oligonucleotides directly to the gold surface. 

The kit contains ready-to-use pre-made mixtures. No activation, manipulation or time consuming "salt-aging" steps are required for conjugation. Simply mix your thiol-modified oligonucleotide with the pre-activated gold nanoparticles supplied in the kit. Conjugation of the oligonucleotide is achieved by the formation of a strong and stable gold-thiol bond. 

Kit Components

  • 3 vials of 50nm OligoREADY™ Gold Nanoparticles (lyophilized) - Sufficient for generation of 3 x 0.2 ml gold conjugate at OD=10

Features & Benefits

  • Results in thiol-oligo bound directly to the gold surface through a stable thiol-gold bond.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Does not require time consuming salt aging procedures saving you valuable research time.

Gold Nanoparticle Specifications

Gold Surface: Proprietary OligoREADY™-coating

Core diameter: 50nm 

Concentration: OD=2 (when dissolved a final volume of 1 ml)

Particles per ml: ~7.02E+10 (when dissolved to a final volume of 1 ml)

Absorbance (lambda max): 535nm

50nm gold nanoparticles UV-VIS spectrum

Diameter  Peak SPR Wavelength (nm) NPS/ml* Wt. Conc. (mg/ml)* Molar Ext. (M-1cm-1) Size Dispersity (+/-nm) Particle Volume (nm3) Surface Area (nm2) Surface/Volume Ratio Particle Mass (g) Molar Mass (g/mol) Molar Concentration*
50 535 3.512E+10 4.45E-02 1.72E+10 <12% 6.54E+04 7.85E+03 0.12 1.27E-15 7.64E+08 5.83E-11

* All concentrations as indicated at an optical density of 1 (OD=1, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)