30nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Scale-Up Kit)

Cytodiagnostics 30nm NHS-activated Gold Nanoparticle Conjugation Kit have been optimized for high efficiency one-step covalent conjugation of proteins and other primary amine-containing ligands to gold nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanoparticles supplied in the kit. 

This MIDI kit contains 1 vial with lyophilized NHS-activated gold nanoparticles for performing one independent scale-up conjugation reaction.

Kit Components

  • 30nm NHS-Activated Gold Nanoparticles (lyophilized) - 1 vial
  • Protein Resuspension Buffer - 1.5ml
  • Reaction Buffer - 1.5ml
  • Quencher Solution - 1.5ml

Features & Benefits

  • Results in covalently bound ligand and more stable conjugate.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
  • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (5kDa PEG-spacer between gold surface and NHS-group)

Core diameter: 30nm 

Concentration: OD=20 (when dissolved to a final volume of 1ml)

Particles per ml: ~3.58E+12 (when dissolved to a final volume of 1ml)

Absorbance (lambda max): 526nm