Cytodiagnostics 10nm NHS-activated Gold Nanoparticle Conjugation Kit have been optimized for high efficiency one-step covalent conjugation of proteins and other primary amine-containing ligands to gold nanoparticles.
The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanoparticles supplied in the kit.
This mini kit contains 3 individual reaction vials with lyophilized NHS-activated gold nanoparticles for performing three independent conjugation reactions.
- 10nm NHS-Activated Gold Nanoparticles (lyophilized) - 3 vials
- Protein Resuspension Buffer - 1.5ml
- Reaction Buffer - 1.5ml
- Quencher Solution - 1.5ml
Features & Benefits
- Results in covalently bound ligand and more stable conjugate.
- Fast and convenient one-step conjugation reaction with no pre-activation requirements.
- Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
- Precisely engineering surface coating that reduces non-specific protein binding in biological assays.
Gold Nanoparticle Specifications
Surface functionality: NHS-ester (~ 1 NHS group/nm2) (5kDa PEG-spacer between gold surface and NHS-group)
Core diameter: 10nm
Concentration: OD=20 (when dissolved to a final volume of 100ul)
Particles per ml: ~1.2E+14 (when dissolved to a final volume of 100ul)
Absorbance (lambda max): 515-520nm