Choose a Silver Nanoparticle Product Based on Your Particular Application
| Application | Silver Nanoparticle Size Range | Surface Chemistry | Benefits |
| Protein Conjugation | 10nm-100nm | Standard (citrate) | Quick |
| NHS | Covalent conjugation to primary amines, increased conjugate stability, less non-specific protein binding. | ||
| Carboxyl | Covalent conjugation to primary amines, increased conjugate stability, less non-specific protein binding. | ||
| Streptavidin | Conjugation to biotinylated ligands. | ||
| Modification with thiolated ligands | 10nm-100nm | Standard (citrate-coated) | Classic starting material, no additional stabilizers added. |
| Oligonucleotide Conjugation | 10nm-20nm | Standard (citrate) | Ideal for conjugation of thiol-modified oligos to small particle sizes (10nm-20nm). Does not work well for larger particles. |
| 10nm-100nm | NHS | Ideal for covalent conjugation of amine-modified oligos. Final conjugate will have a PEG-linker between oligo and silver surface. | |
| Immunoblotting/Western Blot | 10nm-30nm | Secondary Antibody Silver Conjugates | Colorimetric detection. Permanent label |
| Immunohistochemistry | 10nm-40nm | Secondary Antibody Silver Conjugates | High contrast label |
| Cellular Uptake | 30-80nm | Standard (citrate-coated) | Non-specific cellular uptake |
| Darkfield Microscopy | 50nm-100nm | Silver Conjugates | |
| Lateral Flow/Dip-Stick Assays | 20nm-80nm | Standard (citrate-coated) | Ideal for generation of silver conjugates through passive adsorption of antibodies to the silver nanoparticle surface. |
| NHS | Ideal for covalent conjugation of antibodies to silver nanoparticles. | ||
| Silver Conjugates | Pre-made secondary antibody conjugates | ||
| Tumor Targeting | 30nm-80nm | methyl (methoxy)-PEG | Can in some cases be used for passive targeting of certain tumors in vivo. Inert material with low non-specific protein binding in serum. |
| Light Microscopy | 10nm | Silver Conjugates | Ability to label tissue sections for both light and electron microscopy. Alternative to peroxidase and PAP-based stains. Sensitivity can be enhanced with silver enhancement techniques. |
| ELISA | 5nm-30nm | Silver Conjugates | Colorimetric Detection |
