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Gold Nanoparticle Conjugation

Two different strategies are commonly used for conjugation of proteins to gold nanoparticles, i.e. classic passive adsorption, and covalent conjugation. Cytodiagnostics offers convenient kits for conjugation using either one of these strategies, see product categories below.

The classic passive adsorption strategy utilizes the property of non-functionalized (e.g. citrate coated) standard gold nanoparticles to readily bind proteins when the pH of the solution is at or close to the isoelectric point of the protein to be conjugated. This binding is "non-specific” and is governed by ionic, van der Waals, and hydrophobic forces. Although fairly straightforward, this strategy has several drawbacks such as desorption of the conjugated protein over time, and in some cases, partial denaturation or disturbances to the tertiary protein structure which can impact the gold conjugate functionality. 

In contrast, NHS-activated gold nanoparticles allows for covalent conjugation of a protein while also minimizing many of the drawbacks encountered with the passive conjugation strategy discussed above. In many cases, a gold conjugate with an increased functionality and stability is thus obtained.

Covalent conjugation to NHS-activated gold nanoparticles is achieved by the reaction between primary amines available on proteins and the N-hydroxysuccinimide ester functionalized gold surface. Conjugation is quick and straightforward and only requires mixing of your protein of interest with the pre-activated gold nanoparticles supplied with our kits.

Browse these categories under "Gold Nanoparticle Conjugation"


Featured products under "Gold Nanoparticle Conjugation"

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Item #
Description
Price
Qty
CGN5K-5-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 5nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN5K-5-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 5nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN5K-5-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 5nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-5-100
Kit to optimize antibody and protein adsorption to Cytodiagnostics 5nm gold nanoparticles. Complete system with buffers and 100ml of 5nm gold nanoparticles.
$355.00
CGN5K-10-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 10nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN5K-10-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 10nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN5K-10-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 10nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-10-100
Kit to optimize antibody and protein adsorption to Cytodiagnostics 10nm gold nanoparticles. Complete system with buffers and 100ml of 10nm gold nanoparticles.
$355.00
CGN5K-15-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 15nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN5K-15-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 15nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN5K-15-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 15nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-15-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 15nm gold nanoparticles. Complete system with buffers and 100ml of 15nm gold nanoparticles.
$355.00
CGN5K-20-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 20nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN5K-20-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 20nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN5K-20-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 20nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-20-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 20nm gold nanoparticles. Complete system with buffers and 100ml of 20nm gold nanoparticles.
$355.00
CGN5K-30-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 30nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN5K-30-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 30nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN5K-30-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 30nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-30-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 30nm gold nanoparticles. Complete system with buffers and 100ml of 30nm gold nanoparticles.
$355.00
CGN5K-40-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 40nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN5K-40-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 40nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN5K-40-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 40nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-40-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 40nm gold nanoparticles. Complete system with buffers and 100ml of 40nm gold nanoparticles.
$355.00
CGN10K-50-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 50nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN10K-50-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 50nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN10K-50-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 50nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-50-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 50nm gold nanoparticles. Complete system with buffers and 100ml of 50nm gold nanoparticles.
$355.00
CGN10K-60-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 60nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN10K-60-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 60nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN10K-60-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 60nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-60-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 60nm gold nanoparticles. Complete system with buffers and 100ml of 60nm gold nanoparticles.
$355.00
CGN10K-70-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 70nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN10K-70-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 70nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN10K-70-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 70nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-70-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 70nm gold nanoparticles. Complete system with buffers and 100ml of 70nm gold nanoparticles.
$355.00
CGN10K-80-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 80nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN10K-80-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 80nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN10K-80-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 80nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-80-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 80nm gold nanoparticles. Complete system with buffers and 100ml of 80nm gold nanoparticles.
$355.00
CGN10K-90-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 90nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN10K-90-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 90nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN10K-90-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 90nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-90-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 90nm gold nanoparticles. Complete system with buffers and 100ml of 90nm gold nanoparticles.
$355.00
CGN10K-100-1
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 100nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 10ug of protein.
$165.00
CGN10K-100-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 100nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 10ug of protein.
$445.00
CGN10K-100-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 100nm gold nanoparticles. Contains enough reagents for conjugation of 100ug of protein.
$445.00
GO-100-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 100nm gold nanoparticles. Complete system with buffers and 100ml of 100nm gold nanoparticles.
$355.00
GO-LF-200
Kit to optimize protein adsorption to Cytodiagnostics 40nm and 60nm gold nanoparticles for lateral flow applications. Complete system with buffers and 100ml each of 40nm and 60 nm gold nanoparticles.
$525.00
GO-MEM-200
Kit to optimize protein adsorption to Cytodiagnostics 20nm and 30nm gold nanoparticles for membrane blotting applications. Complete system with buffers and 100ml each of 20nm and 30 nm gold nanoparticles.
$525.00
GO-TEM-200
Kit to optimize protein adsorption to Cytodiagnostics 5nm and 10nm gold nanoparticles for TEM applications. Complete system with buffers and 100ml each of 5nm and 10 nm gold nanoparticles.
$525.00