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    • Cytodiagnostics
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    60nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)

    Our Products: Applications > Western Blot and Immunoblotting Products > Nanoparticle Conjugation Kits > Gold Nanoparticle Conjugation > Gold Conjugation Kits for Covalent Conjugation
    60nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
    60nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
    60nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
    60nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
    60nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
    60nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
    60nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
    60nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
    (Click to see larger image and other views)
    SKU: CGN10K-60-3
    One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 60nm gold nanoparticles. Contains enough reagents for conjugation of 200ug of protein.
    Price: $445.00
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    Product Details
    Cytodiagnostics 60nm NHS-activated Gold Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to gold nanoparticles.

    The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanoparticles supplied in the kit. 

    Kit Components

    • 60nm NHS-Activated Gold Nanoparticles (lyophilized)
    • Protein Resuspension Buffer
    • Reaction Buffer
    • Quencher Solution

    Features & Benefits

    • Results in covalently bound ligand and more stable conjugate.
    • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
    • Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
    • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

    Gold Nanoparticle Specifications

    Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between gold surface and NHS-group)
    Core diameter:60nm 
    Concentration: OD=20 (when dissolved to a final volume of 1ml)
    Particles per ml: ~3.92E+11 (when dissolved to a final volume of 1ml)
    Absorbance (lambda max): 540nm

    60nm-Gold-Nanoparticles-UV-VIS

    Diameter

    Peak SPR Wavelength (nm)

    NPS/ml*

    Wt. Conc (mg/ml)*

    Molar Ext (M-1cm-1)

    Size Dispersity (+/-nm)

    Particle Volume (nm3)

    Surface Area (nm2)

    Surface/ Volume Ratio

    Particle Mass (g)

    Molar Mass (g/mol)

    Molar Concentration*

    60

    540

    1.96E+10

    4.30E-02

    3.07E+10

    <10%

    1.13E+05

    1.13E+04

    0.1

    2.19E-15

    1.32E+09

    3.25E-11

    *All concentrations as indicated at an optical density of 1 (OD=1)

      Product Sheet

      MSDS

      Gold Nanoparticle Properties

      Gold Nanoparticle Handling and Storage

      Adsorption of Proteins to Gold Nanoparticles

      Gold Nanoparticle Surface Design
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