Western Blot Products
Western Blot is a sensitive method for detection of small amounts of target antigen transferred or spotted onto a support membrane. Cytodiagnostics horseradish peroxidase (HRP) and fluorescently labeled secondary antibodies are affinity purified and available in several serum adsorbed formats for reduced background and optimal sensitivity when used in conjunction with our chemiluminescent substrates and blocking solution.
Western Blot Detection
Chemiluminescence is a common and sensitive method of detecting minute amounts of antigen transferred or spotted onto a membrane. When the membrane is probed with a horseradish peroxidase (HRP) labeled probe, such as an antibody, and developed using a chemiluminescent substrate, light is emitted which is captured using a imaging system or autoradiography film.
Cytodiagnostics fluorescent probes are ideal for fluorescent detection of target antigen in blotting applications. Fluorescent detection affords additional benefits over traditional chemiluminescent and chromogenic detection. Fluorescent probes improve accuracy, reduce time, reduce cost and can be multiplexed.
Blocking Solutions and Stabilizers
Cytodiagnostics Membrane Blocking Solution is a superior alternative to traditional blocking solutions such as milk, BSA and casein. Traditional blocking solutions can reduce sensitivity and inadequately block the membrane. Cytodiagnostics blocking solution is also well suitable for standard dilution of antibody conjugates such as gold nanoparticle probes.
The HRP stabilizing solution can be used to dilute and store horseradish peroxidase conjugates at working concentrations down to 1 ug/ml while maintaining stability for at least 6 months when stored at 4 C.