1-866-344-3954

80nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)

80nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
80nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
80nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
80nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
80nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
80nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
80nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
80nm NHS-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
(Click to see larger image and other views)
SKU: CGN10K-80-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 80nm gold nanoparticles. Contains enough reagents for conjugation of 200ug of protein.
Price: $445.00
Product Details
Cytodiagnostics 80nm NHS-activated Gold Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to gold nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanoparticles supplied in the kit. 

Kit Components

  • 80nm NHS-Activated Gold Nanoparticles (lyophilized)
  • Protein Resuspension Buffer
  • Reaction Buffer
  • Quencher Solution

Features & Benefits

  • Results in covalently bound ligand and more stable conjugate.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
  • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between gold surface and NHS-group)
Core diameter: 80nm 
Concentration: OD=20 (when dissolved to a final volume of 1ml)
Particles per ml: ~1.57E+11 (when dissolved to a final volume of 1ml)
Absorbance (lambda max): 553nm

80nm-Gold-Nanoparticles-UV-VIS

Diameter

Peak SPR Wavelength (nm)

NPS/ml*

Wt. Conc (mg/ml)*

Molar Ext (M-1cm-1)

Size Dispersity (+/-nm)

Particle Volume (nm3)

Surface Area (nm2)

Surface/ Volume Ratio

Particle Mass (g)

Molar Mass (g/mol)

Molar Concentration*

80

553

7.82E+09

4.06E-02

7.70E+10

<10%

2.68E+05

2.01E+04

0.075

5.20E-15

3.13E+09

1.30E-11

*All concentrations as indicated at an optical density of 1 (OD=1)
Customers Also Viewed
Check the check boxes next to the additional products that you would like to add to the shopping cart, then click on the "Add to Cart" button above. A check box is not shown if a product requires that you select an option or fill out a field. () indicates a required accessory.