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Gold Nanoparticle Selection Guide

Choose Gold Nanoparticle Products Based on Application


Application Gold Nanoparticle
Size Range
Surface Chemistry Benefits Related Documents
Protein Conjugation 5nm-100nm standard (citrate) Quick Classic Passive Adsorption of Proteins to Gold Nanoparticles
NHS Covalent conjugation to primary amines, increased stability, less non-specific protein binding. Conjugation of proteins to NHS-activated gold nanoparticles
Maleimide Covalent conjugation to thiol groups, increased stability, less non-specific protein binding. -
Carboxyl Covalent conjugation, increased stability, less non-specific protein binding. Covalent conjugation of proteins to carboxyl gold nanoparticles
Amine Conjugation of carboxylated ligands -
Streptavidin Can be used with any biotinylated ligand, ideal for high-throughput screenings. -
Modification with thiolated ligands 
(PEG-SH etc.)
5nm-100nm standard (citrate) Classic starting material, no additional stabilizers added -
stabilized (surfactant) Increased stability during functionalization but reduced kinetics -
Oligonucleotide Conjugation 5nm-15nm standard (citrate) Ideal for conjugation of thiolated oligonucleotides to small particle sizes. -
5nm-100nm OligoREADY™ Ideal for conjugation of thiol modified oligos to particles between 5nm-100nm in diameter. -
5nm-100nm Maleimide Ideal for covalent conjugation of thiol modified oligos to particles between 5nm-100nm in diameter. -
5nm-100nm NHS For covalent conjugation of amine functionalized oligonucleotides.
Ideal when a linker is required between the gold surface and conjugated oligonucleotide.
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Immuno-dot blot/Western blot 5nm-20nm Protein conjugated gold nanoparticles (antibodies, streptavidin etc) Colorimetric straightforward detection (no equipment required)
Generates a permanent label
Immunoblotting protocol for gold conjugates
Immunohistochemistry (TEM) 5nm-40nm Protein conjugated gold nanoparticles (antibodies, streptavidin etc) High contrast label -
Flow Cytometry 50nm-400nm Gold Size Standards Ideal for standardization of results between runs and experiments when analyzing particles in the 50nm-400nm range. -
Cellular Uptake 30nm-60nm Transferrin gold conjugate Active uptake through endocytosis -
Standard (citrate) Non-specific cellular uptake -
Cationic gold (available upon request) High efficiency non-specific cellular uptake -
Darkfield Microscopy 50nm-100nm Gold conjugates - -
Lateral Flow/Dip-Stick Assays 30nm-80nm Standard (citrate) Allows for development of rapid testing kit, point of care assays Lateral flow immunoassays
NHS
Carboxyl
Amine
Streptavidin
Protein A
Protein G
Tumor Targeting 30nm-80nm methoxy-PEG Allows for passive targeting of certain tumors in vivo
Inert material with low non-specific protein binding in serum
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Light Microscopy 5nm-10nm Gold secondary antibody conjugates Ability to label tissue sections for both light and electron microscopy. 
Alternative to peroxidase and PAP based stains.
Sensitivity can be enhanced with silver enhancement techniques
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ELISA 5nm-30nm Gold antibody conjugates Straightforward colorimetric detection -