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Gold Nanoparticle Conjugation Optimization Kit, (60nm)

Gold Nanoparticle Conjugation Optimization Kit, (60nm)
Gold Nanoparticle Conjugation Optimization Kit, (60nm)
Gold Nanoparticle Conjugation Optimization Kit, (60nm)
Gold Nanoparticle Conjugation Optimization Kit, (60nm)
Gold Nanoparticle Conjugation Optimization Kit, (60nm)
Gold Nanoparticle Conjugation Optimization Kit, (60nm)
Gold Nanoparticle Conjugation Optimization Kit, (60nm)
Gold Nanoparticle Conjugation Optimization Kit, (60nm)
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Item #: GO-60-100
Kit to optimize antibody or protein adsorption to Cytodiagnostics 60nm gold nanoparticles. Complete system with buffers and 100ml of 60nm gold nanoparticles.
Price: $355.00
Product Details
Supplied in Kit:

  • 60nm standard gold nanoparticles (100ml, conc. OD1)
  • 12 conjugation optimization buffers (1.5ml each)
  • 10% NaCl (100ml)
  • Protein resuspension buffer
  • Detailed conjugation optimization protocol

Kit contains enough material to optimize 10 protein conjugates, or one optimization reaction and scale-up production of 20ml of conjugate at a final optical density of OD3.

Note: Only use with Cytodiagnostics standard  or reactant free gold nanoparticles.

Gold nanoparticle conjugates have been used for a wide range of biological applications including the use as probes in light and electron microscopy (TEM) and for membrane blotting (immunogold dot-blot).


 

Stable gold nanoparticle conjugates can readily be prepared by passive adsorption of a protein to the gold nanoparticle surface, which is mediated by electrostatic and hydrophobic interactions between the protein and the surface layer of the colloidal gold.


Our gold nanoparticle protein conjugation optimization kits are supplied as complete and easy to use systems for increased success rates in the development of good quality gold nanoparticle conjugates.




Diameter

Peak SPR Wavelength (nm)

NPS/ml*

Wt. Conc (mg/ml)*

Molar Ext (M-1cm-1)

Size Dispersity (+/-nm)

Particle Volume (nm3)

Surface Area (nm2)

Surface/ Volume Ratio

Particle Mass (g)

Molar Mass (g/mol)

Molar Concentration*

60

540

1.96E+10

4.30E-02

3.07E+10

<10%

1.13E+05

1.13E+04

0.1

2.19E-15

1.32E+09

3.25E-11

*All concentrations as indicated at an optical density of 1 (OD=1)

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