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90nm NHS-Activated Gold NanoUrchins Conjugation Kit (MIDI Kit)

90nm NHS-Activated Gold NanoUrchins Conjugation Kit (MIDI Kit)
90nm NHS-Activated Gold NanoUrchins Conjugation Kit (MIDI Kit)
90nm NHS-Activated Gold NanoUrchins Conjugation Kit (MIDI Kit)
90nm NHS-Activated Gold NanoUrchins Conjugation Kit (MIDI Kit)
90nm NHS-Activated Gold NanoUrchins Conjugation Kit (MIDI Kit)
90nm NHS-Activated Gold NanoUrchins Conjugation Kit (MIDI Kit)
90nm NHS-Activated Gold NanoUrchins Conjugation Kit (MIDI Kit)
90nm NHS-Activated Gold NanoUrchins Conjugation Kit (MIDI Kit)
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SKU: GUN10K-90-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 90nm gold nanourchins. Contains enough reagents for conjugation of 100ug of protein.
Price: $445.00
Product Details
Cytodiagnostics 90nm NHS-activated Gold NanoUrchins Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to gold nanourchins.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanourchins is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanourchins supplied in the kit. 

Kit Components

  • 90nm NHS-Activated Gold NanoUrchins (lyophilized)
  • Protein Resuspension Buffer
  • Reaction Buffer
  • Quencher Solution

Features & Benefits

  • Results in covalently bound ligand and more stable conjugate.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Spacer between the gold surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
  • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between gold surface and NHS-group)
Core diameter: 90nm 
Concentration: OD=20 (when dissolved to a final volume of 100ul)
Particles per ml: ~1.07E+11 (when dissolved to a final volume of 100ul)
Absorbance (lambda max): 630nm



90nm Gold NanoUrchins UV-VIS Spectrum

Diameter

Peak SPR Wavelength (nm)

NPS/ml*

Wt. Conc (mg/ml)*

Molar Ext (M-1cm-1)

Size Dispersity (+/-nm)

Particle Volume (nm3)#

Surface Area (nm2)#

Surface/ Volume Ratio#

Particle Mass (g)

Molar Mass (g/mol)

Molar Concentration*

90

630

5.37E+09

3.97E-02

1.12E+11

<8%

3.82E+05

2.54E+04

0.067

7.40E-15

4.46E+09

8.92E-12

*All concentrations as indicated at an optical density of 1 (OD=1), # Data is approximated based upon a spherical nanoparticle.
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