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5nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
5nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
5nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
5nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
5nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
5nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
5nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
5nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (MIDI Kit)
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SKU: MG5K-5-3
One-Step MIDI scale-up kit for covalent conjugation of proteins and other thiol-containing ligands to 5nm gold nanoparticles. Contains enough reagents for conjugation of 2mg of protein.
Price: $445.00
Product Details

Cytodiagnostics 5nm Maleimide-Activated Gold Nanoparticle Conjugation Kit have been optimized for high efficiency one-step conjugation of thiol-containing ligands to gold nanoparticles.


The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated maleimide gold nanoparticles supplied in the kit. 


Kit Components


  • 5nm Maleimide-Activated Gold Nanoparticles (lyophilized)
  • Protein Resuspension Buffer
  • Reaction Buffer
  • Quencher Solution


Features & Benefits


  • Results in covalently bound ligand and more stable conjugate.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
  • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.


Gold Nanoparticle Specifications


Surface functionality: Maleimide (~ 1 maleimide group/nm2) (5kDa PEG-spacer between gold surface and maleimide-group)

Core diameter: 5nm 

Concentration: OD=20 (when dissolved to a final volume of 100ul)

Particles per ml: ~1.1E+14 (when dissolved to a final volume of 100ul)

Absorbance (lambda max): 515-520nm


5nm-Gold-Nanoparticles-UV-VIS

Diameter

Peak SPR Wavelength (nm)

NPS/ml*

Wt. Conc (mg/ml)*

Molar Ext (M-1cm-1)

Size Dispersity (+/-nm)

Particle Volume (nm3)

Surface Area (nm2)

Surface/ Volume Ratio

Particle Mass (g)

Molar Mass (g/mol)

Molar Concentration*

5

515-520

5.47E+13

6.94E-02

1.10E+07

<15%

6.54E+01

7.85E+01

1.2

1.27E-18

7.64E+05

9.08E-08

*All concentrations as indicated at an optical density of 1 (OD=1)