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50nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)

50nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
50nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
50nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
50nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
50nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
50nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
50nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
50nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
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SKU: CGN10K-50-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 50nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 20ug of protein.
Price: $445.00
Product Details
Cytodiagnostics 50nm NHS-activated Gold Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to gold nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanoparticles supplied in the kit. 

Kit Components

  • 50nm NHS-Activated Gold Nanoparticles (lyophilized)
  • Protein Resuspension Buffer
  • Reaction Buffer
  • Quencher Solution

Features & Benefits

  • Results in covalently bound ligand and more stable conjugate.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
  • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between gold surface and NHS-group)
Core diameter: 50nm 
Concentration: OD=20 (when dissolved to a final volume of 100ul)
Particles per ml: ~7.02E+11 (when dissolved to a final volume of 100ul)
Absorbance (lambda max): 535nm

50nm-Gold-Nanoparticles-UV-VIS

Diameter

Peak SPR Wavelength (nm)

NPS/ml*

Wt. Conc (mg/ml)*

Molar Ext (M-1cm-1)

Size Dispersity (+/-nm)

Particle Volume (nm3)

Surface Area (nm2)

Surface/ Volume Ratio

Particle Mass (g)

Molar Mass (g/mol)

Molar Concentration*

50

535

3.51E+10

4.45E-02

1.72E+10

<10%

6.54E+04

7.85E+03

0.12

1.27E-15

7.64E+08

5.83E-11

*All concentrations as indicated at an optical density of 1 (OD=1)
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