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40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)
40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)
40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)
40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)
40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)
40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)
40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)
40nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)
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SKU: SN5K-40-2
One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 40nm silver nanoparticles. Contains enough reagents for conjugation of 10 x 20ug of protein.
Price: $445.00
Product Details
Cytodiagnostics 40nm NHS-activated Silver Nanoparticle Conjugation Kit have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied with the kit. 

Kit Components

  • 40nm NHS-Activated Silver Nanoparticles (lyophilized)
  • Protein Resuspension Buffer
  • Reaction Buffer
  • Quencher Solution

Features & Benefits

  • Results in covalently bound ligand and more stable conjugate.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Spacer between the silver nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
  • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between silver surface and NHS-group)
Core diameter: 40nm 
Concentration: OD=50 (when dissolved to a final volume of 100ul)
Particles per ml: ~1.1E+12 (when dissolved to a final volume of 100ul)
Absorbance (lambda max): 405-425nm

40nm-Silver-Nanoparticles-UV-Vis

Diameter

Peak SPR Wavelength (nm)

NPS/ml*

Wt. Conc (mg/ml)*

Size Dispersity (+/-nm)

Particle Volume (nm3)

Surface Area (nm2)

Surface/ Volume Ratio

Particle Mass (g)

Molar Mass (g/mol)

Molar Concentration*

40

405-425

~5.7E+10

2.00E-02

<15%

3.35E+04

5.03E+03

0.15

3.52E-16

2.12E+08

9.47E-11


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