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    • Cytodiagnostics
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    20nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)

    Our Products: Applications > Western Blot and Immunoblotting Products > Nanoparticle Conjugation Kits > Gold Nanoparticle Conjugation > Gold Conjugation Kits for Covalent Conjugation
    20nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
    20nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
    20nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
    20nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
    20nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
    20nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
    20nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
    20nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 reactions)
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    SKU: CGN5K-20-2
    One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 20nm gold nanoparticles. Contains enough reagents for conjugation of 10 x 40ug of protein.
    Price: $445.00
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    Product Details
    Cytodiagnostics 20nm NHS-activated Gold Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to gold nanoparticles.

    The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanoparticles supplied in the kit. 

    Kit Components

    • 20nm NHS-Activated Gold Nanoparticles (lyophilized)
    • Protein Resuspension Buffer
    • Reaction Buffer
    • Quencher Solution

    Features & Benefits

    • Results in covalently bound ligand and more stable conjugate.
    • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
    • Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
    • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

    Gold Nanoparticle Specifications

    Surface functionality: NHS-ester (~ 1 NHS group/nm2) (5kDa PEG-spacer between gold surface and NHS-group)
    Core diameter: 20nm 
    Concentration: OD=20 (when dissolved to a final volume of 100ul)
    Particles per ml: ~1.3E+13 (when dissolved to a final volume of 100ul)
    Absorbance (lambda max): 524nm

    20nm-Gold-Nanoparticles-UV-VIS

    Diameter

    Peak SPR Wavelength (nm)

    NPS/ml*

    Wt. Conc (mg/ml)*

    Molar Ext (M-1cm-1)

    Size Dispersity (+/-nm)

    Particle Volume (nm3)

    Surface Area (nm2)

    Surface/ Volume Ratio

    Particle Mass (g)

    Molar Mass (g/mol)

    Molar Concentration*

    20

    524

    6.54E+11

    5.31E-02

    9.21E+08

    <12%

    4.19E+03

    1.26E+03

    0.3

    8.12E-17

    4.89E+07

    1.09E-09

    *All concentrations as indicated at an optical density of 1 (OD=1)

      Product Sheet

      MSDS

      Gold Nanoparticle Properties

      Gold Nanoparticle Handling and Storage

      Adsorption of Proteins to Gold Nanoparticles

      Gold Nanoparticle Surface Design
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