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15nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (3 reactions)
15nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (3 reactions)
15nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (3 reactions)
15nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (3 reactions)
15nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (3 reactions)
15nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (3 reactions)
15nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (3 reactions)
15nm Maleimide-Activated Gold Nanoparticle Conjugation Kit (3 reactions)
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SKU: MG5K-15-1
One-Step mini kit for covalent conjugation of proteins and other thiol-containing ligands to 15nm gold nanoparticles. Contains enough reagents for conjugation of 3 x 80 ug of protein.
Price: $165.00
Product Details

Cytodiagnostics 15nm Maleimide-Activated Gold Nanoparticle Conjugation Kit has been optimized for high efficiency one-step conjugation of thiol-containing ligands to gold nanoparticles.


The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated maleimide gold nanoparticles supplied in the kit. 


Kit Components


  • 15nm Maleimide-Activated Gold Nanoparticles (lyophilized)
  • Protein Resuspension Buffer
  • Reaction Buffer
  • Quencher Solution


Features & Benefits


  • Results in covalently bound ligand and more stable conjugate.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements.
  • Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
  • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.


Gold Nanoparticle Specifications


Surface functionality: Maleimide (~ 1 maleimide group/nm2) (5kDa PEG-spacer between gold surface and maleimide-group)

Core diameter: 15nm 

Concentration: OD=20 (when dissolved to a final volume of 100ul)

Particles per ml: ~3.28E+13 (when dissolved to a final volume of 100ul)

Absorbance (lambda max): 520nm

15nm-Gold-Nanoparticles-UV-VIS

Diameter

Peak SPR Wavelength (nm)

NPS/ml*

Wt. Conc (mg/ml)*

Molar Ext (M-1cm-1)

Size Dispersity (+/-nm)

Particle Volume (nm3)

Surface Area (nm2)

Surface/ Volume Ratio

Particle Mass (g)

Molar Mass (g/mol)

Molar Concentration*

15

520

1.64E+12

5.61E-02

3.67E+08

<12%

1.77E+03

7.07E+02

0.4

3.43E-17

2.06E+07

2.72E-09

*All concentrations as indicated at an optical density of 1 (OD=1)