Cyto™ Fluorescent Dyes
Cytodiagnostics fluorescent Cyto™ dyes are available with excitation and emission wavelengths spanning the visible and infrared spectrum and are great alternatives to other traditional dyes on the market.
These dyes are available in a variety of chemistries (NHS, maleimide, carboxyl and amine) and can be covalently coupled and conjugated to amino acids, proteins, antibodies, hormones, sugars, dNTPs, oligonucleotides or other affinity tags (e.g. biotin or streptavidin) with high efficiency. This will allow you to obtain your required probes or markers for sensitive in vivo imaging and other cell and molecular biological applications.
Our Cyto™ fluorescent dyes are also available pre-conjugated to a wide range of secondary antibodies and gold nanoparticles.
Click to select a fluorescent dye based on your excitation source
NHS Ester Dyes
NHS (N-hydroxysuccinimide) esters are commonly used for fluorescent labeling of amine-groups in proteins, oligonucleotides and other ligands.
Available with absorption maximum from 350nm to 840nm.
Featured Product - Cyto 488 - NHS ester (1mg)
Absorption/Emission max: 490nm/516nm
Molar Absorbance: 73000 M-1cm-1
Great alternative to Alexa Fluor 488™
Near Infrared Dyes
Near-infrared fluorescent dyes (NIR), i.e. dyes with emission maximum above 680nm, are ideal labels for in vivo imaging.
Available with absorption maximum from 680nm to 840nm.
Featured Product - Cyto 750 - NHS ester (1mg)
Absorption/Emission max: 748nm/772nm
Molar Absorbance: 270000 M-1cm-1
Great alternative to Alexa Fluor 750 and Cy7.
Our Near Infrared Labels
| Cyto Label | Absorption maximum | Emission maximum | Alternative to | Laser excitation source |
|---|---|---|---|---|
| Cyto 750 | 747  |
776  |
Alexa Fluor 750, Cy7 | Far-red diode lasers; xenon-arc lamp; dye-pumped lasers |
| Cyto 770 | 771 |
801 |
xenon-arc lamp; dye-pumped laser | |
| Cyto 780 | 783 |
800 |
xenon-arc lamp; dye-pumped laser | |
| Cyto 840 | 844 |
884 | xenon-arc lamp; dye-pumped laser |
Large Stokes Shift Dyes
Large stokes shift (LSS) dyes are ideal for multicolor labeling and FACS applications.
Available with absorption maximum from 500nm to 520nm and emission from 590nm-664nm.
Featured Product - Cyto 500LSS Large Stokes Shift Dye - NHS ester (1mg)
Absorption/Emission max: 500nm/630nm
Molar Absorbance: 50000 M-1cm-1
Can be used together with our Cyto 488 labeled reagents for multiplexing.
Our LSS Labels
| Cyto Label | Absorption maximum | Emission maximum | Laser excitation source |
|---|---|---|---|
| Cyto 500LSS | 500  |
630  |
Argon 488 laser |
| Cyto 510LSS | 509 |
590  |
Argon 488 laser |
| Cyto 514LSS | 515 |
650 |
Argon 514 laser |
| Cyto 520LSS | 520 |
664 |
Argon 514 laser |
Maleimide Dyes
Malemide fluorescent dyes are sulfhydryl-reactive reagents that can be used for labeling of free thiol(-SH)-groups in for example proteins, oligonucleotides and other ligands at pH 7.0-7.5.
Available with absorption maximum from 350nm to 840nm.
Featured Product - Cyto 610 - maleimide (1mg)
Absorption/Emission max: 610nm/630nm
Molar Absorbance: 80000 M-1cm-1
Great alternative to Texas Red™
Carboxylic Acid Dyes
Carboxyl acid functionalized dyes can be used for labeling of amine groups.
Available with absorption maximum from 350nm to 840nm.
Featured Product - Cyto 840 - carboxylic acid (1mg)
Absorption/Emission max: 844nm/884nm
Molar Absorbance: 220000 M-1cm-1
Ideal for in vivo imaging
Amine Dyes
Amine functionalized dyes can be used for labeling of carboxyl groups.
Available with emission from 350nm to 840nm.
Featured Product - Cyto 488 - amino-modified
Absorption/Emission max: 490nm/516nm
Molar Absorbance: 73000 M-1cm-1
Great alternative to Alexa Fluor 488
Conjugation to Fluorescent Dyes
Custom conjugation of your protein or antibody to any of our Cyto™ Fluorescent Dyes with absorption maximum from 350nm-840nm.
Our service include:
- Conjugation of 1-5mg of protein.
- Purification of your protein conjugate using size exclusion cromatography.
- Characterisation of your labeled protein.
- Fluorescent properties including emission and absorption spectra.
- Determination of fluorescent dye to protein ratio.
- Determination of the concentration of the final conjugate.
Price(USD): $550/1-5mg* of Labeled Protein
*1mg minimum quantity.
Video
Our Cyto Fluorescent Dyes by Laser Excitation Source
| Cyto Label | Absorption maximum | Emission maximum | Alternative to | Laser excitation source |
|---|---|---|---|---|
| Cyto 350 | 354  |
431  |
Alexa Fluor 350, AMCA, AMCA-X | Argon laser; UV-light |
| Cyto 405 | 400 |
420 |
Alexa Fluor 405, Pacific Blue, Cascade Blue | Blue diode laser; Violet 405 krypton laser |
| Cyto 415 | 418 |
467  |
Blue diode laser; Violet 405 krypton laser | |
| Cyto 488 | 490  |
516 |
Fluorescein (FITC), Alexa Fluor 488, Cy2 | Argon 488 blue-green laser |
| Cyto 505 | 505 |
530 |
RhodamineGreen | Argon 488 blue-green laser |
| Cyto 532 | 539 |
561  |
Alexa Fluor 532, Tetramethylrhodamine (TMR, TRITC) | Nd:YAG laser |
| Cyto 546 | 551 |
572  |
Alexa Fluor 546, Tetramethylrhodamine (TMR) | Helium-Neon 543 green laser |
| Cyto 555 | 557 |
574 |
Alexa Fluor 555, Cy3, Tetramethylrhodamine (TMR) | Helium-Neon 543 green laser |
| Cyto 590 | 581 |
598 |
TexasRed, Alexa Fluor 568, Cy3.5 | Helium-Neon 594 laser; Ar-Kr mixed-gas laser |
| Cyto 610 | 610  |
630 |
Texas Red | Helium-Neon 594 laser |
| Cyto 633 | 637 |
657 |
Alexa Fluor 633 | Helium-Neon 633 laser; Red diode 635 laser |
| Cyto 647 | 653 |
672 |
Alexa Fluor 647, Cy5, Allophycocyanin | Krypton-Argon 647 laser |
| Cyto 670 | 673 |
694 |
Alexa Fluor 660, Alexa Fluor 680, Cy5.5 | Helium-Neon laser |
| Cyto 680 | 690 |
709 |
Alexa Fluor 680, Cy5.5 | Helium-Neon laser |
| Cyto 700 | 704 |
723 |
Alexa Fluor 700 | Far-red diode lasers; xenon-arc lamp; dye-pumped laser |
| Cyto 750 | 747 |
776 |
Alexa Fluor 750, Cy7 | Far-red diode lasers; xenon-arc lamp; dye-pumped lasers |
| Cyto 770 | 771 |
801 |
xenon-arc lamp; dye-pumped laser | |
| Cyto 780 | 783 |
800 |
xenon-arc lamp; dye-pumped laser | |
| Cyto 840 | 844 |
884 | xenon-arc lamp; dye-pumped laser |
Our Large Stokes Shift Labels
| Cyto Label | Absorption maximum | Emission maximum | Laser excitation source |
|---|---|---|---|
| Cyto 500LSS | 500 |
630 |
Argon 488 laser |
| Cyto 510LSS | 509 |
590 |
Argon 488 laser |
| Cyto 514LSS | 515 |
650 |
Argon 514 laser |
| Cyto 520LSS | 520 |
664 |
Argon 514 laser |
Our Near Infrared Labels
| Cyto Label | Absorption maximum | Emission maximum | Alternative to | Laser excitation source |
|---|---|---|---|---|
| Cyto 750 | 747 |
776 |
Alexa Fluor 750, Cy7 | Far-red diode lasers; xenon-arc lamp; dye-pumped lasers |
| Cyto 770 | 771 |
801 |
xenon-arc lamp; dye-pumped laser | |
| Cyto 780 | 783 |
800 |
xenon-arc lamp; dye-pumped laser | |
| Cyto 840 | 844 |
884 | xenon-arc lamp; dye-pumped laser |
